Journal: Diabetes

Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes

doi: 10.2337/db14-0929

Figure Lengend Snippet: NOD.Ncf1m1J islets exhibit lowered M1 and elevated M2 levels of macrophage markers. Purified islet whole-cell lysates from prediabetic 16-week-old NOD and NOD.Ncf1m1J mice were used in an immunoblot analysis for P-STAT6 (Y641) and STAT6 (A), iNOS (B), and Arg-1 (C). Densitometry statistics for Western blotting represent the average of three experiments. ***P < 0.001; *P < 0.05. WT, wild type.

Article Snippet: For Western blotting, whole-cell lysates were probed with antibodies against iNOS (Novus Biologicals), Arg-1, STAT6 (Cell Signaling), P-STAT6 (Y641) (BD Bioscience), or β-actin (Sigma-Aldrich) as described ( 18 ).

Techniques: Purification, Western Blot

Journal: British Journal of Nutrition

Article Title: Treatment with oligonol, a low-molecular polyphenol derived from lychee fruit, attenuates diabetes-induced hepatic damage through regulation of oxidative stress and lipid metabolism

doi: 10.1017/s0007114511001322

Figure Lengend Snippet: Fig. 2. NF-kBp65 (A), cyclo-oxygenase-2 (COX-2) (B) and inducible NO synthase (iNOS) (C) expressions in the liver. m/m, Misty; db/db, diabetic; Veh, db/db vehicle-treated mice; Oligo-10, db/db mice treated with oligonol at 10 mg/kg body weight; Oligo-20, db/db mice treated with oligonol at 20 mg/kg body weight. Values are means (n 6 or n 10), with standard errors represented by vertical bars. a,b Mean values with unlike letters were significantly different (P,0·05; Duncan’s test).

Article Snippet: Rabbit polyclonal antibodies against PPARa, SREBP-1, SREBP-2, NF-kBp65 and RAGE, and mouse monoclonal antibody against cyclo-oxygenase-2 (COX-2) and inducible NO synthase (iNOS) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques:

Journal: British Journal of Nutrition

Article Title: Treatment with oligonol, a low-molecular polyphenol derived from lychee fruit, attenuates diabetes-induced hepatic damage through regulation of oxidative stress and lipid metabolism

doi: 10.1017/s0007114511001322

Figure Lengend Snippet: Fig. 5. Hepatic mRNA expressions of acetyl-CoA carboxylase (ACC) (A), fatty acid synthase (FAS) (B) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) (C). m/m, Misty; db/db, diabetic; Veh, db/db vehicle-treated mice; Oligo-10, db/db mice treated with oligonol at 10 mg/kg body weight; Oligo-20, db/db mice treated with oligonol at 20 mg/kg body weight. Values are means (n 6 or n 10), with standard errors represented by vertical bars. a,b Mean values with unlike letters were significantly different (P,0·05; Duncan’s test).

Article Snippet: Rabbit polyclonal antibodies against PPARa, SREBP-1, SREBP-2, NF-kBp65 and RAGE, and mouse monoclonal antibody against cyclo-oxygenase-2 (COX-2) and inducible NO synthase (iNOS) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques:

Journal: Acta Pharmacologica Sinica

Article Title: Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis

doi: 10.1038/aps.2012.129

Figure Lengend Snippet: Photomicrographs of representative iNOS-positive and 3-nitrotyrosine-positive stains in gingival tissue from patients with chronic periodontitis (immunohistochemical staining): (A) countless iNOS+ PMNs and monocytes (scale bar, 50 μm); (B) decreased iNOS+ cells in the SRP+placebo group (scale bar, 50 μm); (C) sparse iNOS+ cells in SRP+SDD group (scale bar, 50 μm); (D) countless 3NT+ PMNs and monocytes (scale bar, 100 μm); (E) decreased 3NT+ cells in the SRP+placebo group (scale bar, 50 μm); (F) sparse 3NT+ cells in the SRP+SDD group (scale bar, 50 μm). This figure is representative of 3 measurements (×400). iNOS+=induced nitric oxide synthase positive cells; 3NT+=3-nitrotyrosine positive cells; SRP=scaling and root planing; SDD=subantimicrobial dose of doxycycline.

Article Snippet: The following primary monoclonal antibodies were used: anti iNOS (R&D Systems, RD-MAB9502, MN, USA), anti 3-nitrotyrosine (3NT) (Novus Europe, KA0445, ABNOVA, Cambridge, UK).

Techniques: Immunohistochemical staining, Staining

Journal: Acta Pharmacologica Sinica

Article Title: Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis

doi: 10.1038/aps.2012.129

Figure Lengend Snippet: Periodontal immunohystochemical and serum nitrosative stress parameters of the patients.

Article Snippet: The following primary monoclonal antibodies were used: anti iNOS (R&D Systems, RD-MAB9502, MN, USA), anti 3-nitrotyrosine (3NT) (Novus Europe, KA0445, ABNOVA, Cambridge, UK).

Techniques: